Publications　since 2001

Smith BN, Topp SD, Fallini C, Shibata H, Chen HJ, Troakes C, King A, Ticozzi N, Kenna KP, Soragia-Gkazi A, Miller JW, Sato A, Dias DM, Jeon M, Vance C, Wong CH, de Majo M, Kattuah W, Mitchell JC, Scotter EL, Parkin NW, Sapp PC, Nolan M, Nestor PJ, Simpson M, Weale M, Lek M, Baas F, Vianney de Jong JM, Ten Asbroek ALMA, Redondo AG, Esteban-Pérez J, Tiloca C, Verde F, Duga S, Leigh N, Pall H, Morrison KE, Al-Chalabi A, Shaw PJ, Kirby J, Turner MR, Talbot K, Hardiman O, Glass JD, De Belleroche J, Maki M, Moss SE, Miller C, Gellera C, Ratti A, Al-Sarraj S, Brown RH Jr, Silani V, Landers JE, Shaw CE.

Sci Transl Med. 2017 May 3;9(388):eaad9157

The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein.

Kanadome T, Shibata H, Kuwata K, Takahara T, Maki M.

FEBS J. 2017 Jan;284(1):56-76.

The calcium-binding protein ALG-2 regulates protein secretion and trafficking via interactions with MISSL and MAP1B proteins.

Takahara T, Inoue K, Arai Y, Kuwata K, Shibata H, Maki M.

J Biol Chem. 2017 Oct 13;292(41):17057-17072.

２０１６

Multifaceted Roles of ALG-2 in Ca(2+)-Regulated Membrane Trafficking. (Review)

Maki M, Takahara T, Shibata H.

Int J Mol Sci. 2016 Aug 26;17(9):1401.

２０１５

Tubby-like protein superfamily member PLSCR3 functions as a negative regulator of adipogenesis in mouse 3T3-L1 preadipocytes by suppressing induction of late differentiation stage transcription factors
Inokawa A, Inuzuka T, Takahara T, Shibata H, Maki M.
Biosci Rep. 2015 Dec 16;36(1). pii: e00287.

Structural Analysis of the Complex between Penta-EF-Hand ALG-2 Protein and Sec31A Peptide Reveals a Novel Target Recognition Mechanism of ALG-2.
Takahashi T, Kojima K, Zhang W, Sasaki K, Ito M, Suzuki H, Kawasaki M, Wakatsuki S, Takahara T, Shibata H, Maki M.
Int J Mol Sci. 2015 Feb 6;16(2):3677-99.

A New Role for Annexin A11 in the Early Secretory Pathway via Stabilizing Sec31A at the Endoplasmic Reticulum Exit Sites (ERES).
Shibata H, Kanadome T, Sugiura H, Yokoyama T, Yamamuro M, Moss SE, Maki M.
J Biol Chem. 2015 Feb 20; 2290(8):4981-93.

２０１４
Involvement of calpain-7 in epidermal growth factor receptor degradation via the endosomal sorting pathway.
Maemoto Y, Ono Y, Kiso S, Shibata H, Takahara T, Sorimachi H, Maki M.
FEBS J. 2014 Aug;281(16):3642-55.

２０１３
Nuclear ALG-2 Protein Interacts with Ca2+ Homeostasis Endoplasmic Reticulum Protein (CHERP) Ca2+-dependently and Participates in Regulation of Alternative Splicing of Inositol Trisphosphate Receptor Type 1 (IP3R1) Pre-mRNA.

Sasaki-Osugi K, Imoto C, Takahara T, Shibata H, Maki M.
J Biol Chem. 2013 Nov 15;288(46):33361-75.

VPS37 Isoforms Differentially Modulate the Ternary Complex Formation of ALIX, ALG-2, and ESCRT-I.
Okumura M, Katsuyama AM, Shibata H, Maki M.
Biosci Biotechnol Biochem. 2013 Aug 23;77(8):1715-21.

Identification of phosphorylation sites in the C-terminal region of charged multivesicular body protein 1A (CHMP1A).
Maemoto Y, Shibata H, Maki M.
Biosci Biotechnol Biochem. 2013;77(6):1317-9.

Analysis of limited proteolytic activity of calpain-7 using non-physiological substrates in mammalian cells.
Maemoto Y, Kiso S, Shibata H, Maki M.
FEBS J. 2013 Jun;280(11):2594-607.

ALG-2-interacting Tubby-like protein superfamily member PLSCR3 is secreted by exosomal pathway and taken up by recipient cultured cells.
Inuzuka T, Inokawa A, Chen C, Kizu K, Narita H, Shibata H, Maki M.
Biosci Rep. 2013 Jan 26.

Mammalian ESCRT-III-Related Protein IST1 Has a Distinctive Met-Pro Repeat Sequence That Is Essential for Interaction with ALG-2 in the Presence of Ca(2+).
Okumura M, Takahashi T, Shibata H, Maki M.

Biosci Biotechnol Biochem. 2013 May 23;77(5):1049-54.

Hyper-activation of the target of rapamycin (Tor) kinase 1 decreases intracellular glutathione content in Saccharomyces cerevisiae as revealed by LC-MS/MS analysis.

Oku M, Ichiki Y, Shiraishi A, Takahara T, Maeda T, Sakai Y.

Biosci Biotechnol Biochem. 2013;77(7):1608-11.

Changes in cell morphology are coordinated with cell growth through the TORC1 pathway.

Goranov AI, Gulati A, Dephoure N, Takahara T, Maeda T, Gygi SP, Manalis S, Amon A.

Curr Biol. 2013 Jul 22;23(14):1269-79.

Evolutionarily conserved regulation of TOR signalling. (Review)

Takahara T, Maeda T.

J Biochem. 2013 Jul;154(1):1-10.

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Prediction of a New Ligand-Binding Site for Type 2 Motif Based on the Crystal Structure of ALG-2 by Dry and Wet Approaches
Takahashi T, Suzuki H, Inuzuka T, Shibata H, Maki M.

Int. J. Mol. Sci. 2012, 13, 7532-7549

Identification of the P-body component PATL1 as a novel ALG-2-interacting protein by in silico and Far-Western screening of proline-rich proteins.

Osugi K, Suzuki H, Nomura T, Ariumi Y, Shibata H, Maki M.

J Biochem. 2012 Jun;151(6):657-66.

ESCRT and calpain - old and new relationships.*

FEBS J. 2012 Apr;279(8):1398.

Evolutionary and physical linkage between calpains and penta-EF-hand Ca(2+) -binding proteins. *

Maki M, Maemoto Y, Osako Y, Shibata H.

FEBS J. 2012 Apr;279(8):1414-21.

Stress granules: the last refuge of TORC1? (Review)

Takahara T, Maeda T.

Cell Cycle. 2012 Oct 15;11(20):3707-8.

TORC1 of fission yeast is rapamycin-sensitive.

Takahara T, Maeda T.

Genes Cells. 2012 Aug;17(8):698-708.

Transient sequestration of TORC1 into stress granules during heat stress.

Takahara T, Maeda T.

Mol Cell. 2012 Jul 27;47(2):242-52

２０１１
Structure and function of ALG-2, a penta-EF-hand calcium-dependent adaptor protein.

Maki M, Suzuki H, Shibata H.

Sci China Life Sci. 2011 Aug;54(8):770-9.

Hepatitis C virus hijacks P-body and stress granule components around lipid droplets.
Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, Ikeda M, Kato N.
J Virol. 2011 Jul;85(14):6882-92.

Calpain-7 binds to CHMP1B at its second α-helical region and forms a ternary complex with IST1 .

Maemoto Y, Osako Y, Goto E, Nozawa E, Shibata H, Maki M.

J Biochem. 2011 Oct;150(4):411-21.

The ESCRT system is required for hepatitis C virus production.
Ariumi Y, Kuroki M, Maki M, Ikeda M, Dansako H, Wakita T, Kato N.
PLoS One. 2011 Jan 11;6(1):e14517.

２０１０
Autolytic activity of human calpain 7 is enhanced by ESCRT-III-relatedprotein IST1 through MIT-MIM interaction.
Osako Y, Maemoto Y Tanaka R, Suzuki H, Shibata H, Maki M.
FEBS J 2010 77, (21), 4412-4426

The ALG-2 Binding Site in Sec31A Influences Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
Shibata H, Inuzuka T, Yoshida H, Sugiura H, Wada I, and Maki M
Biosci. Biotech. Biochem. 2010 Sep 23;74(9):1819-26.

Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2ΔGF122) and structural roles of F122 in target recognition
Inuzuka T, Suzuki H, Kawasaki M, Shibata M, Wakatsuki S, and Maki M,
BMC Structural Biology BMC Struct Biol. 2010 Aug 6;10:25 (page 1-14) Free access

Distinct functions of human MVB12A and MVB12B in the ESCRT-I dependent on their posttranslational modifications. Tsunematsu T, Yamauchi E, Shibata H, Maki M, Ohta T, Konishi H.

Biochem Biophys Res Commun. 2010 Aug 20;399(2):232-7. Epub 2010 Jul 21. Erratum in: Biochem Biophys Res Commun. 2011 Mar 4;406(1):158-9.

２００９

Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101 .
Okumura M, Ichioka F, Kobayashi R, Suzuki H, Yoshida H, Shibata H, Maki M.
Biochem Biophys Res Commun. 2009 Aug 14;386(1):237-241.

The mechanism of Ca2+-dependent recognition of Alix by ALG-2: insights from X-ray crystal structures . (Review)
Suzuki H, Kawasaki M, Inuzuka T, Okumura M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M.
Biochem Soc Trans. 2009 Feb;37(Pt 1):190-194.

２００８
Crystalization and X-ray diffraction analysis of N-terminally truncated human ALG-2
Suzuki H, Kawasaki M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):974-977.

Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism.
Suzuki H, Kawasaki M, Inuzuka T, Okumura M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M.
Structure. 2008 Oct 8;16(10):1562-1573.

Human calpain 7/PalBH associates with a subset of ESCRT-III-related proteins in its N-terminal region and partly localizes to endocytic membrane compartments.
Yorikawa C, Takaya E, Osako Y, Tanaka R, Terasawa Y, Hamakubo T, Mochizuki Y, Iwanari H, Kodama T, Maeda T, Hitomi K, Shibata H, Maki M.,

J Biochem. 2008 Jun;143(6):731-45

Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4)
Fumitaka Ichioka1, Ryota Kobayashi, Keiichi Katoh, Hideki Shibata and Masatoshi Maki
FEBS J. 2008 Feb;275(4):682-692.

Adaptor protein Ruk/CIN85 is associated with a subset of COPI coated membranes of the Golgi complex.
Havrylov S,Ichioka F, Powell K, Borthwick EB, Baranska J, Maki M, Buchman VL.
Traffic.9(5):798-812 (2008).

Identificationof alix-type and non-alix-type ALG-2-binding sites in human phospholipidscramblase 3: Differential binding to an alternatively spliced isoform andamino acid-substituted mutants .
Shibata H, Suzuki H, Kakiuchi T, Inuzuka T, Yoshida H, Mizuno T, Maki M.
J Biol Chem. 2008 Apr 11;283(15):9623-32.

２００７
The Penta-EF-hand Protein ALG-2 and Its Interacting Proteins . (Review)
Maki, M. and Shibata, H.
Calcium Binding Proteins 2, 4-10 (2007)

ALG-2 directly binds Sec31A and localizes at endoplasmic reticulum exit sites in a Ca2+-dependent manner.

Shibata H, Suzuki H, Yoshida H, Maki M.
Biochem Biophys Res Commun. 2007 Feb 16;353(3):756-763.

HD-PTP and Alix share some membrane-traffic related proteins that interact with their Bro1 domains or proline-rich regions .
Ichioka F, Takaya E, Suzuki H, Kajigaya S, Buchman VL, Shibata H, Maki M.
Arch Biochem Biophys. (2007) 457(2):142-9.

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CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway.
Horii M, Shibata H, Kobayashi R, Katoh K, Yorikawa C, Yasuda J, Maki M.
Biochem J. (2006) 400, 23-32.

２００５
The penta-EF-hand protein ALG-2 directly interacts with the ESCRT-I componentTSG101 and Ca2+ 2+-dependently colocalizes to aberrant endosomes with dominant-negativeAAA ATPase SKD1/Vps4B.
Katoh K, Suzuki H, Terasawa Y, Mizuno T, Yasuda J, Shibata H, Maki M.
Biochem. J. (2005) 391(Pt 3):677-685

Reevaluation of the predicted gene structure of Dictyostelium cystatin A3 (cpiC) by the nucleotide sequence determination of its cDNA and its phylogenetic position in cystatin superfamily .
El-Halawany, M. S., Shibata, H., Hitomi, K., and Maki, M.
Mol. Biol. Rep. 32, 257-264 (2005)

Distinct mechanistic roles of calpain and caspase activation in neurodegeneration as revealed in mice overexpressing their specific inhibitors.
Higuchi M, Tomioka M, Takano J, Shirotani K, Iwata N, Masumoto H, Maki M, Itohara S, Saido TC.　
J Biol Chem. 280, 15229-15237 (2005)

Calpain mediates excitotoxic DNA fragmentation via mitochondrial pathways in adult brains: Evidence from calpastatin-mutant mice.
Takano J, Tomioka M, Tsubuki S, Higuchi M, Iwata N, Itohara S, Maki M, Saido TC.
J Biol Chem. 280, 16175-16184 (2005)

Overexpression of calpastatin inhibits L8 myoblast fusion.
Barnoy S, Maki M, Kosower NS.
Biochem Biophys Res Commun. 2005 Jul 8;332(3):697-701.

Identification of Rab GTPase-activating protein-like protein (RabGAPLP) as a novel Alix/AIP1-interacting protein.
Ichioka, F., Horii, M., Katoh, K., Terasawa, Y., Shibata, H., and Maki, M.
Biosci. Biotechnol. Biochem. 69, 861-865 (2005)

Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting.
Yorikawa, C., Shibata, H., Waguri, S., Hatta, K., Horii, M., Katoh, K., Kobayashi, T., Uchiyama, Y., and Maki, M.
Biochem. J. 387, 17-26 (2005)

Dictyostelium discoideum requires an Alix/AIP1 homolog, DdAlix, for morphogenesis in alkaline environments
Ohkouchi, s., Saito, H., Aruga, F., Maeda, T., Shibata, H., and Maki, M.
FEBS Lett. 579, 1745-1750 (2005)

２００４
DdAlix, an Alix/AIP1 homolog in Dictyostelium discoideum, is required for multicellular development under low Ca2+ conditions
Ohkouchi, S., El-Halawany, M. S., Aruga, F., Shibata, H., Hitomi, K., and Maki, M.
Gene 337, 131-139 (2004)

Identification of cysteine protease inhibitors that belong to cystatin family 1 in the cellular slime mold Dictyostelium discoideum.
El-Halawany MS, Ohkouchi S, Shibata H, Hitomi K, Maki M.
Biol. Chem. 385, 547-550 (2004)

CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms.
katoh, K., Shibata, H., Hatta, K. and Maki, M.
Arch. Biochem. Biophys. 421, 159-165 (2004)

The penta-EF-hand protein ALG-2 interacts with a region containing PxY repeats in Alix/AIP1, which is required for the subcellular punctate distribution of the amino-terminal truncation form of Alix/AIP1.
Shibata, H., Yamada, K., Mizuno, T., Yorikawa, C., Takahashi, H., Satoh, H., Kitaura, Y., and Maki, M.
J. Biochem. 135, 117-128 (2004)

２００３
The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting.
Katoh, K., Shibata, H., Suzuki, H., Nara, A., Ishidoh, K., Kominami, E., Yoshimori, T., and Maki, M.
J. Biol. Chem. 278, 39104-39113 (2003)

Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement.
Potter DA, Srirangam A, Fiacco KA, Brocks D, Hawes J, Herndon C, Maki M, Acheson D, Herman IM.
J Biol Chem. 2003 Aug 8;278(32):30403-12. Epub 2003 May 22.

A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor.
Todd B, Moore D, Deivanayagam CC, Lin GD, Chattopadhyay D, Maki M, Wang KK, Narayana SV.
J Mol Biol. 2003 Apr 18;328(1):131-46.

２００２
Structures, functions and molecular evolution of the penta-EF-hand Ca 2+-binding proteins. *
Maki, M., Kitaura, Y., Satoh, H., Ohkouchi, S., and Shibata, H.
Biochim. Biophys. Acta 1600, 51-60 (2002)

The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexinXI in a Ca2+-dependent manner.
Satoh, H., Nakano, Y., Shibata, H., and Maki, M.
Biochim. Biophys. Acta 1600, 61-67 (2002)

Both ALG-2 and peflin, penta-EF-hand (PEF) proteins, are stabilized by dimerization through their fifth EF-hand regions.
Kitaura, Y., Satoh, H., Takahashi, H., Shibata, H., and Maki, M.
Arch. Biochem. Biophys. 399, 12-18 (2002)

２００１
Peflin and ALG-2, members of the penta-EF-hand protein family, form a heterodimer that dissociates in a Ca2+-dependent manner.
Kitaura, Y., Matsumoto, S., Satoh, H., Hitomi, K., and Maki, M.
J. Biol. Chem. 276, (2001)

Publications since 2001

Publications　since 2001